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Example text
1977, 99, 2564–2571. If we regard supramolecular chemistry in its simplest sense as involving some kind of (non-covalent) binding or complexation event, we must immediately define what is doing the binding. In this context we generally consider a molecule (a ‘host’) binding another molecule (a ‘guest’) to produce a ‘host–guest’ complex or supermolecule. Commonly the host is a large molecule or aggregate such as an enzyme or synthetic cyclic compound possessing a sizeable, central hole or cavity.
E. the average number of occupied binding sites, in this case on the metal, M. 27) m 1 + ∑ βi [ L] i i =1 Where βi represents the stepwise stability constants and [L] is the concentration of free ligand. e. 30) equations. 30) A Scatchard plot is thus a plot of r/[L] as a function of r and appears as a straight line for non-cooperative systems, a convex curve for negative cooperativity and a concave curve for positive cooperativity. A Hill plot is a plot of log[r/(m Ϫ r)] vs. log[L]. Cooperativity results in two straight lines connected by a S-shaped curve.
In a spectrophotometric experiment absorbance at a properly chosen wavelength is usually directly proportional to complex concentration. 12) Fluorescence Titration Fluorescence titration measurements are based on the proportion of fluorescence intensity to fluorophore concentration (concentration of fluorescent species in solution; this is often a fluorescent guest, G). 5 Job plot for a 1:1 host–guest complex. where kG and k11 represent proportionality constants for the guest and the 1:1 host–guest complex respectively.