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The Molecular Repertoire of Adenoviruses II: Molecular by P. C. Van der Vliet (auth.), Walter Doerfler, Petra Böhm

By P. C. Van der Vliet (auth.), Walter Doerfler, Petra Böhm (eds.)

For a long time this virus procedure has served--and maintains to do so--to pioneer investigations at the molecular biology, biochemistry and genetics of mammalian mobilephone platforms. This 3 quantity paintings provides an updated account of modern simple study in a single of an important experimental structures for biochemical, mobilephone organic, genetic, virological and epidemiological investigations in mammalian molecular biology. during this, the second one of 3 volumes, the eye is grew to become to such themes as DNA replication, recombination and integration, and post-transcriptional regulate. The chapters were written through a world staff of top specialists of their respective fields of interest.

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Studies on pTP have been facilitated by the cloning of cDNA copies of the gene (PETIIT et al. 1988) into vaccinia virus (STUNNENBERG et al. 1988) or baculovirus (BOSHER et al. 1990) vectors for high-level expression in human or insect cells. T. Hay et al. Adenovirus pTP is processed to TP via an intermediate form by the action of the virus-coded protease, which is produced at high levels late in the infectious cycle and is essential for viral infectivity. Sequence alignments and site-directed mutagenesis of the protease indicate that the enzyme may represent a new subclass of cysteine protease (RANCOURT et al.

37 6 Cellular Proteins Involved in Adenovirus DNA Replication ........................ 1 Nuclear Factor I ......................................................... 2 Nuclear Factor III ........................................................ 41 41 42 7 A Model for the Initiation of Adenovirus DNA Replication. . . . . . . . . . . . 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . .

Nucleic Acids Res 17: 8917-8929 Mul YM, Verrijzer CP, Van der Vliet PC (1990) Transcription factors NFl and NFIII/oct-1 function independently, employing different mechanisms to enhance adenovirus DNA replication. J Virol 64: 5510-5518 Nagata K, Guggenheimer RA, Enomoto T, Lichy JH, Hurwitz J (1982) Adenovirus DNA replication in vitro: identification of a host factor that stimulates synthesis of the preterminal protein-dCMP complex. Proc Natl Acad Sci USA 79: 6438-6442 Nagata K, Guggenheimer RA, Hurwitz J (1983) Adenovirus DNA replication in vitro: synthesis of fulllength DNA with purified proteins.

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