By M. M. Rapport (auth.), Rodolfo Paoletti, Paul M. Vanhoutte, Nicoletta Brunello, Franco M. Maggi (eds.)
This quantity represents the lawsuits ofthe invited lectures ofthe first foreign Symposium on "Serotonin from cellphone Biology to Pharmacology and Therapeutics" which was once held in Florence on March 29 -Aprill, 1989. This assembly, held below the co-sponsorship of the Serotonin membership and the Fondazione Giovanni Lorenzini, represents the 1st try to assemble scientists desirous about the complexity of the motion of 5-hydroxytryptamine through the physique and in numerous species. consequently this quantity offers the reader with the original assessment of the assets, results, receptors, physiological activities and pathological position of Serotonin. As such it is going to be of curiosity not just to the individual devoting herorhis study efforts to the learn of 5-hydroxytryptamine but additionally to all students or even clinicians desirous to understand how the strong monoamine can modulate mobile services. To speed up the e-book of those complaints the Editors and the publishers have chosen the digicam prepared structure and feature kept away from a long refereeing procedure. consequently the clinical content material of, and the critiques expressed within the chapters are the only real accountability of the authors. The Editors Milan and Houston The Editors are looking to thank Mrs. H. Liepman and her employees at Kluwer for the instructed and effective dealing with of the manuscripts.
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Extra info for Serotonin: From Cell Biology to Pharmacology and Therapeutics
Sample text
Buspirone. and ipsapirone. along with other drugs. such as RU 24969 and methysergide. were inactive. All of the tested 5-HT antagonists except ICS 205 930 were inactive in competing with 1 pM 5-HT. which corresponds to ten times the EC50. These included spiperone. methiothepin. metergoline. mesu1ergine. ketanserin. (-)pindo101. MDL 72222. and cocaine. The observed KI of ICS 205 930 (1 pM) precluded the classification of this receptor as the 5-HT3 subtype (see [6]). In view of the unique pharmacological profile of this receptor.
Between receptors and the fIrst enzyme(s) activated on stimulation, is mediated by guanine nucleotide binding proteins (G-proteins). The G-proteins are composed of 3 subunits, ex, Band yand are associated with the plasma membrane. As we discussed earlier [23] the availability of these subunits might determine the extent to which phospholipase C is activated upon receptor stimulation. Amplificatory effects at the level of signal transduction on addition of two agonists as we found in our experiments are explained when the activation of one receptor would lead to an increase of G-proteins for the second receptor.
Serotonin: From Cell Biology to Pharmacology and Therapeutics, 19-24. © 1990 by Kluwer Academic Publishers. 20 2. 1. CELL CULTURE COS-7 and HeLa cells were grown as rnonolayers in DMEM (Gibco) supplemented with 10% fetal calf serum, penicillin (100 units/ml) and streptomycin (100 mg/ml), in 5 % C02 at 37'C. 2. Radioligand binding studies 5-HT1AR binding studies were carried out with [3Hj8-0H-DPAT as previously described (8). 3. Expression vector and cell transfection The expression vector pBC121G-21 was constructed as described (8).