By Amersham Biosciences
Read Online or Download Protein Separations' Handbook Collection PDF
Best nonfiction_5 books
Management: Meeting and Exceeding Customer Expectations, 9th Edition
Administration: assembly AND EXCEEDING purchaser expectancies, 9th version is a finished survey of the foundations and practices of administration as they're being utilized around the globe. The content material and contours are dependent to augment carrying on with subject matters which are woven into the chapters' narratives: 1) the endless attempt through managers and firms to fulfill or exceed buyer wishes, and a couple of) the necessity of corporations and their humans to be guided by way of powerful management.
- Elephant and Ivory Trade in Myanmar
- Modeling, Design, and Simulation of Systems with Uncertainties
- Working with Preferences: Less Is More (Cognitive Technologies) by Souhila Kaci (2011-06-24)
- Where skies are not cloudy (Texas Poets Series, No 4)
- Controlled Genetic Manipulations (Neuromethods, v65)
- Illinois Classical Studies, Volume II, 1977.
Extra info for Protein Separations' Handbook Collection
Example text
20 illustrates how a three step increase in eluting strength can be used to obtain maximum resolution of the fraction of interest (shaded peak). In the first step, the strength and the volume of the elution buffer is optimized to elute all compounds binding less strongly to the gel than the compound of interest. The elution strength and volume of buffer should be large enough to elute these contaminating weaker binding substances, but it must not exceed that level where the peak of interest starts to co-elute with the contaminating compounds.
Increased salt concentration will also give increased costs which may be of importance if the HIC step is to be a part of a manufacturing process. Finally, not much can be gained by changing the pH of the equilibration buffer since the resolution obtained was considered to be satisfactory. Next step: Continue with method development as outlined under ‘‘Optimizing a HIC step’’. If low binding capacity is a problem and problems with increased salt concentration as outlined above are encountered – TRY A MEDIUM WITH A DIFFERENT LIGAND or, if available, A MEDIUM WITH A HIGHER DEGREE OF LIGAND SUBSTITUTION.
These parameters include: type of buffer salt, salt concentration, buffer pH, temperature, bed height, flow rate, gradient shape and gradient slope. The solute As in other adsorption chromatography techniques, the way HIC is used depends on the size of the solute molecule. Small molecules such as small peptides interact with the medium by single point attachment. Their migration velocity depends directly on the binding constant of a single bond and can vary over a wide interval depending on the ionic strength of the mobile phase.