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Progress in Molecular and Subcellular Biology by H. Stebbings (auth.), J. Jeanteur, Y. Kuchino, Professor Dr.

By H. Stebbings (auth.), J. Jeanteur, Y. Kuchino, Professor Dr. Werner E. G. MÜller, P. L. Paine (eds.)

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Here, the restricting factor is not the cytoskeleton, but a particular RNA species. Nucleo-Cytoplasmic Transport of mRNA 29 RNA-protein interactions playa different kind of role in snRNP accumulation in nuclei. When U1, U2, U4, US or U6 snRNA are injected into oocytes they become highly concentrated in the nucleus, unlike tRNA and 7S RNA, which remain in the cytoplasm (De Robertis etal. 1982). Specific association of these snRN~ with proteins is crucial for the rapid transport and accumulation.

1984) showed that tRNA processing and nucleocytoplasmic transport are affected independently. One nucleotide, G-57, was particularly crucial for transport: replacement of it by C resulted in a 50-fold decrease in transport rate (Zasloff 1983). Generally, the first 62 nucleotides in the molecule seem to contain the sequences regulating transcription, processing and transport (Adeniyi-Jones etal. 1984). Nucleocytoplasmic transport of tRNA involves a specific facilitated diffusion mechanism, showing saturability, sequence-specificity, and competition between tRNAs (Zasloff 1983).

Because living cells have large ATP pools and at least some capacity for anaerobic metabolism, the energy-dependence of transport processes cannot be ascertained by any available techniques. This is true not least of amphibian oocytes. 4. Intracellular homeostasis precludes the possibility of constraining transport systems in the living cell in the kinds of ways that would usefully elucidate mechanisms (e. g. by altering the ionic conditions or introducing toxic inhibitors during experiments with long time-courses).

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