By Richard Flavell (auth.), J.P. Gustafson, Peter Langridge, Daryl J. Somers (eds.)
Plant genomics is a transforming into and continuously evolving box of research, one that has received a lot floor in previous years during the improvement of complex learn and knowledge administration instruments. In Plant Genomics: tools and Protocols, professional researchers discover the present matters and methodologies of this increasing box, particularly addressing parts of gene discovery and the practical research of genes with a spotlight at the basic instruments and sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling. Chapters hire intriguing new the right way to examine molecular plant breeding expertise and gene sensible research through transformation, mutation, protein functionality, and gene expression. Composed within the hugely profitable Methods in Molecular Biology series structure, each one bankruptcy includes a short advent, step by step equipment, an inventory of priceless fabrics, and a Notes part which stocks tips about troubleshooting and heading off recognized pitfalls.
Comprehensive and leading edge, Plant Genomics: equipment and Protocols is an important consultant for all plant scientists who're drawn to extra stories within the zone of genomics study.
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8, 195–202. Y. R. (1999) A general approach to single nucleotide polymorphism discovery. Nat. Genet. 23, 452–456. Chagné, D. , Batley, J. W. A. E. N. , eds. ), Springer, NY, 77– 94. Chapter 3 Genetic Maps and the Use of Synteny Chris Duran, David Edwards, and Jacqueline Batley Summary Genetic linkage maps represent the order of known molecular genetic markers along a given chromosome for a given species. This provides an insight into the organisation of a plant genome. In comparative genomics, synteny is the preserved order of genes on chromosomes of related species which results from descent from a common ancestor.
And Karp, A. (1996) Microsatellite libraries enriched for several microsatellite sequences in plants. Biotechniques 20, 758–760. , and Edwards, D. (2004) Simple sequence repeat marker loci discovery using SSRPrimer. Bioinfomatics 20, 1475–1476. , New Technologies for Ultra-High Throughput Genotyping in Plants 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. , and Edwards, D. (2006) SSR Primer and SSR Taxonomy Tree: Biome SSR discovery. Nucleic Acids Res. 34, W656–W659. A. (2003) The International HapMap Project: The International HapMap Consortium.
10, 330–343. Olivier, M. (2005) The Invader® assay for SNP genotyping. Mutat. Res. 573, 103–110. R. (2002) High-throughput genotyping of single nucleotide polymorphisms using new biplex invader technology. Nucleic Acids Res. 30, e53. , Bayan. , and Thompson, S. (2008) A non-PCR-based Invader® assay quantitatively detects single-copy genes in complex plant genomes. Mol. Breeding 21, 173–181. S. (2003) Highly parallel SNP genotyping. Cold Spring Harb. Symp. Quant. Biol. 68, 69–78. S. (2005) A genome-wide scalable SNP genotyping assay using microarray technology.