By A. Engler
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Fingerprints observed in lanes (a) and (l) are identical with each probe, suggesting a close relationship of these two samples. RFLPs in organellar DNA can be visualized by one of three experimental strategies.
However, one can choose between a variety of marker systems, each of which combines some — or even most — of the above-mentioned characteristics. 1 Protein Markers and Allozymes For the generation of molecular markers based on protein polymorphisms, the most frequently used technique is the electrophoretic separation of proteins, followed by specific staining of a distinct protein subclass. Less commonly, specific proteins are detected by monoclonal antibodies with an attached fluorescent label.
Willow warbler Gyllensten et al. GGGGACAGGGGACACCC... willow warbler Gyllensten et al. CTATACAGGGCTGGTT... GCCTTTCCCGAG... GAGGGTGGXGGXTCT... M13 phage Vassart et al. GGAGGAGGAAGGGGAGAGGAAGGAGGT... AGGATGGCATGGAGGTGGAGGAGGACATGGCGG... Arabidopsis Tourmente et al. TATTATTATTAGTATA... Orchis chloroplast Cafasso et al. TATTTAATTGCGTTGCTCGACCAACGGGAGAGG... Beta mitochondrion Nishizawa et al. 2 Examples of (mostly GC-rich) minisatellite repeat units cloned from various organisms. The last two lines exemplify AT-rich minisatellite repeat units detected in plant cpDNA and mtDNA, respectively.