PCR in Bioanalysis (Methods in Molecular Biology Vol 92) by Meltzer, Stephen J

By Meltzer, Stephen J

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Cellular debris are then removed by centrifugation (10 min at 1000g). The DNA concentration of the purified viral preparation is determined spectrophotometrically or by ethidium bromide fluorescent quantitation (11). Postive control viral preparations should be diluted at least 1:10 in sterile water prior to addition to the PCR reaction vial or the Tris-EDTA may inhibit DNA amplification. 2. To remove any potential DNA contamination, two sets of instruments are allowed to soak in a 1:10 dilution of bleach for at least 20 min prior to use.

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Rodent parvovirus infections. Lab. Anim. Sci. 46, 370–380. 6. Collins, M. , Jr. and Parker, J. C. (1972) Murine virus contaminants of leukemia viruses and transplantable tumors. J. Natl. Cancer Inst. 49, 1139–1143. 7. , and Meyer, B. (1993) Contamination of transplantable tumors, cell lines, and monoclonal antibodies with rodent viruses. Lab. Anim. Sci. 43, 296–300. 8. , and Pintel, D. (1991) Detection of rodent parvoviruses (MVM, H-1, and RV) by polymerase chain reaction. AALAS Bull. 30, 24 (abstract P06).

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