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Mucins: Methods and Protocols by Karine Rousseau, Dallas M. Swallow (auth.), Michael A.

By Karine Rousseau, Dallas M. Swallow (auth.), Michael A. McGuckin, David J. Thornton (eds.)

Epithelial mucins are huge complicated cellphone floor and secreted glycoproteins produced by way of mucosal epithelial cells. In, Mucins: equipment and Protocols expert researchers within the box element some of the tools that are now standard to review Mucins. those comprise tools and methods for the simplest techniques to analysing each one particular region of mucin biochemistry, body structure and biophysics sooner than offering person designated experimental protocols including troubleshooting and interpretation assistance. Written within the hugely profitable Methods in Molecular Biology™ sequence layout, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, simply reproducible laboratory protocols, and key tips about troubleshooting and averting recognized pitfalls.

Authoritative and practical, Mucins: equipment and Protocols is designed to be an invaluable source for these getting into the mucin box and to facilitate these already learning mucins to develop their experimental ways to knowing mucosal biology.

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Two distinct mutations in the mouse Muc2 gene, obtained by N-ethyl-N-nitrosourea mutagenesis, were found to have a profound effect on the MUC2 mucin (40). The mutations are located in the 5¢ and 3¢ cysteine-rich domains of Muc2 leading to improper processing of Muc2 which failed to be secreted and resulted in the accumulation of Muc2 precursor (the non-glycosylated form) in the ER, causing ER stress. Mice with this mutation had a phenotype similar to ulcerative colitis in humans. 4. MUC2 shows two TR domains, the larger one containing conserved 69-bp repeats and upstream from that a smaller one with poorly conserved 48-bp repeats.

0, and homogenise using four to five strokes of a Dounce homogenizer with a tight pestle. (c) Bring the suspension to 3 mM Mg2+ by adding 30 mM MgCl2 and 100 mM NaCl and centrifuge (1,000 × g, 1 min). (d) Collect supernatant and centrifuge (10,000 × g, 10 min). (e) Collect supernatant and centrifuge (100,000 × g, 90 min). Retain pellet containing membrane fragments. 5% sodium deoxycholate or (3) 6 M guanidinium chloride extraction buffer and incubate on ice for 30 min. 1 Ti rotor, 1 h) and retain the supernatant (see Note 11).

And Chen, Y. (2010) Cloning and Characterization of Human MUC19 Gene. Am J Respir Cell Mol Biol In Press. 47. , Chanock, S. , Chow, W. , and Ye, W. (2010) A comprehensive analysis of common genetic variation in MUC1, MUC5AC, MUC6 genes and risk of stomach cancer. Cancer Causes Control 21, 313–321. 26 K. M. Swallow 48. Ubell, M. , and Kerschner, J. E. (2009) Mucin gene polymorphisms in otitis media patients. Laryngoscope 120, 132–138. 49. Kwon, J. , Lee, S. , Ahn, E. , Seol, S. , Kim, M. , Kim, S.

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