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Microautoradiography and Electron Probe Analysis: Their by W. O. Abel, P. Dörmer, W. Eschrich, E. Fritz, R. G.

By W. O. Abel, P. Dörmer, W. Eschrich, E. Fritz, R. G. Herrmann, A. Läuchli, U. Lüttge, J. B. Passioura, J. D. Pickett-Heaps (auth.), U. Lüttge (eds.)

In organic literature, numerous definitions of quantitative autoradio­ graphy are given. The time period is outlined as both the choice and com­ parison of the density of silver grains above a number of buildings or less than various stipulations, or the decision of absolute amounts of radio­ task. In either those instances, photometric size serves for extra quick and extra distinct overview of grain densities than will be attainable through visible counting of the grains. The apparatus regularly used for the photometric size of silver grains comprises a microscope, a photocell, an digital amplifier method and a reveal unit. Grains could be made obtainable to photometric overview via different types of microscopic illumination: 1. Substage bright-field illumination. 2. Substage dark-field illumination. three. Incident dark-field illumination. four. Vertical bright-field illumination. With these kinds of sorts of illumination, the connection among the luminous flux I absorbed by means of the movie, scattered into the target and mirrored or diffracted, and the flux 10 which isn't suffering from the movie is used as a degree of grain density. given that those are differential measurements, the sunshine beam I transmitted through the movie is in itself a degree of grain density if the luminous flux 10 incident at the grains is stored consistent. This procedure has been utilized in a great number of measuring setups.

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PELC, S. : Automatic grain counter for assessing quantitatively highresolution autoradiographs. Nature 172, 992-993 (1953). : Grain size determination and other applications of the callier effect. J. Soc. Mot. Pict. Eng. 30, 181-190 (1938). : Der zeitliche Ablauf von Protein- und Kohlenhydratsynthesen in der Oogenese bei Apis mellificia L. Verh. Dtsch. Zoo!. Ges. 243-251 (1965). EVANS, T. : Selection of radioautographic technique for problems in biology. Nucleonics 3, 52-59 (1948). : Uber die Abhangigkeit der gemessenen Substanzmengen von der Fokussierung.

Acta Radiol. Suppl. 93, 1-123 (1952). : Matrix theory for quantitative evaluation of auto radiographs. Lab. Invest. 8,113-126 (1959). PELC, S. : Quantitative aspects of autoradiography. Exp. , Suppl. 4, 231-237 (1957). PELC, S. : On the question of automatic or visual grain counting. Lab. Invest. 8, 127-130 (1959). PERRY, R. : Quantitative autoradiography. In: D. M. ), Methods in cell physiology, vol. I, p. 305-326. New York: Academic Press 1964. PODDAR, R. : On the quantitative relation between isotopic beta radiation and its photographic response.

Diameter of measuring aperture approx. ~ iOO I u .... ill ill -0 75 ~ 2 ill E o ~ 50 > Cii ~ 25 1-25)Jm-l cause the grains were focused in white light and measured in blue light. The stray-light component was therefore considerably larger than in the focused measurement illustrated in Fig. 13. If accumulations of grains are to be measured, as is the case, for example, with 3H-labeled cell nuclei, the measuring aperture should always be chosen slightly larger than the area actually covered by the grains.

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