By M. Hegner, W. Grange (auth.), Wolfgang A. Linke, Henk Granzier, Miklós S. Z. Kellermayer (eds.)
A consultant cross-section of elastic biomolecules is roofed during this quantity, which mixes seventeen contributions from top examine teams. cutting-edge molecular mechanics experiments are defined facing the pliancy of DNA and nucleoprotein complexes, titin and titin-like proteins in muscle, in addition to proteins of the cytoskeleton and the extracellular matrix.
The e-book speaks relatively to telephone biologists, biophysicists, or bioengineers, and to senior researchers and graduate scholars alike, who're attracted to contemporary advances in single-molecule know-how (optical tweezers procedure, atomic strength microscopy), EM imaging, and laptop simulation techniques to review nanobiomechanics. The findings mentioned right here have redefined our view of the position mechanical indications play in mobile features and feature drastically helped increase our knowing of organic elasticity in general.
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Printed in the Netherlands. 397 Optical tweezers stretching of chromatin LISA H . POPE* , MARTIN L. BENNINK and JAN GREVE Biophysical Techniques, Department of Applied Physics and MESA + Research Institute, University of Twente , PO Box 217, 7500 AE Enschede, The Netherlands Abstract Recently significant success has emerged from excitmg research involv ing chromatin stretching using optical tweezers . These experiments, in which a single chromatin fibre is attached by one end to a micron-sized bead held in an optical trap and to a solid surface or second bead via the other end , allows manipulation and force detect ion at a single-molecule level.
Thom a F and Brubak er JM (1985) Chromatin reconstituted from tand emly repeated cloned DNA fragment s and core histones: a model system for study of higher ord er structure. Cell 42: 799-8 08. Smith SB, Cui Y and Bustamante C (1996) Overstretchin g B-DNA : the elastic respo nse of individual doubl e- stra nded and single-stra nded DNA molecules. Sc ience 271: 795-7 99. Smith SB, Finzi L and Bustam ante C (1992) Direct mechanica l measurement s of tbe elasticity of single DNA molecules by using magnetic bead s.
Shlyakhtenko LS, Potaman VN, Sinden RR and Lyubchenko YL (1998) Structure and dynamics of supercoil-stab ilized DNA cruciforms. J M ol BioI 280: 61-72 . Simpson RT. Thom a F and Brubak er JM (1985) Chromatin reconstituted from tand emly repeated cloned DNA fragment s and core histones: a model system for study of higher ord er structure. Cell 42: 799-8 08. Smith SB, Cui Y and Bustamante C (1996) Overstretchin g B-DNA : the elastic respo nse of individual doubl e- stra nded and single-stra nded DNA molecules.