By W. John Lockyer
Booklet via Lockyer, W.J.
Read or Download Essentials of ABO–Rh Grouping and Compatibility Testing. Theoretical Aspects and Practical Application PDF
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Additional resources for Essentials of ABO–Rh Grouping and Compatibility Testing. Theoretical Aspects and Practical Application
Example text
While the main purpose of the urgent match is to exclude any major incompatibility, its shortened incubation time will mean that the possibility of detecting those antibodies with low titre and poor avidity is reduced. The technology involved in both normal and urgent compatibility testing is basically the same, the only difference being that the incubation times are shortened to lOmin from the usual 45-60 min. This shortened incubation time is compensated for by lightly centrifuging each test before reading.
The majority of the R h - D negative will also be negative for C and E and will therefore be of the Rh genotype cde/cde. Approximately 2 per cent will, however, group as D negative, C or E positive. These bloods will be of the genotype Cde/cde or cdE/cde and should be kept to one side in the blood bank as they can be used for transfusion to either R h - D positive recipients or in some selected cases to Rh-negative recipients, particularly when there is a shortage of cde/cde genotyped blood. D o n o r bloods grouping as Rh B L O O D G R O U P SYSTEM 4 u 31 negative with anti-D but positive with a n t i - D ' must be treated as RhD positive and placed in the Rh positive blood bank (see p.
In the case of an Rh-negative woman with anti-D, a full Rh genotype of the father's red cells will determine his most probably genotype and give some guidance as to whether or not he is likely to be homozygous for the D antigen (D/D), in which case the fetus will, with a high degree of certainty, be known to have the D antigen and therefore to be incompatible with the maternal antibody, or possibly heterozygous for D (D/d) in which case there would be a 50 Rh B L O O D G R O U P SYSTEM 39 per cent chance of a D-positive fetus and a 50 per cent chance of a Dnegative (d/d) fetus being carried by the mother.