By H. P. Schwan (auth.), Eberhard Neumann, Arthur E. Sowers, Carol A. Jordan (eds.)
Cells should be humorous. try and develop them with a marginally flawed recipe, they usually flip over and die. yet hit them with an electrical box robust adequate to knock over a horse, they usually do sufficient issues to justify foreign conferences, to fill a large booklet, and to guide one to talk of a wholly new expertise for phone manipulation. The very improbability of those occasions not just increases questions on why issues occur but in addition ends up in an extended checklist of functional structures during which the applying of robust electrical fields may perhaps allow the merger of telephone contents or the creation of alien yet very important fabric. necessarily, the fundamental questions and the sensible purposes won't continue in step. The questions are intrinsically tricky. it truly is not easy adequate to research the motion of the fairly vulnerable fields that rotate or align cells, however it is sort of very unlikely to foretell responses to the cell-shredding bursts of electrical energy that make them fuse or to speak in confidence very huge molecular assemblies. however, theoretical reviews and systematic exam of version platforms have produced a few creditable effects, rules which may still finally supply tricks of what to attempt next.
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Additional resources for Electroporation and Electrofusion in Cell Biology
Example text
381-444. Fuhr, G. , 1985, Ueber die Rotation dielektrischer Koerper in rotierenden Feldern, Dissertation, HumboldtUniversitat, Berlin, DDR. , 1986, Rotation of dielectrics in a rotating high frequency field; model experiments and theoretical explanation of the rotation effect of living cells, Biophys. J. 49:395-402. , 1985, The influence of diffusion on the permittivity of a suspension of spherical particles with insulating shells in an electrolyte, J. Phys. D. 18:1891-1896. , 1986. Electrorotation of single cells-A new method for assessment of membrane properties.
This torque acts against the friction in the liquid and establishes a constant spinning in the steady state. The spinning rate as a function of the frequency of the external rotating field gives information on a number of biological parameters, such as the age of the cell, the position in the cell cycle, the physiologic state (live or dead, healthy or poisoned by different noxes), and the type of the cell. It is influenced by several physical parameters of the experiment, such as the chosen frequency, the field strength, the viscosity and conductivity of the surrounding liquid, the presence of cations and anions of different valence, the cell density, and the pH value.
1985, Alignment of microscopic particles in electric fields and its biological implications, Biophys. J. 467:513-518. , 1982, Electric field-mediated fusion and related electrical phenomena, Biochim. Biophys. Acta 694:227-277. , and Arnold, W. , 1983, The interpretation and use of the rotation of biological cells, in: Coherent Excitations in Biological Systems (H. Froehlich and F. ), Springer-Verlag, Berlin, pp. 211-221. Chapter 2 Cellular Spin Resonance Ingolf Lamprecht and Maja Mischel 1. INTRODUCTION Among the different effects living cells exhibit under the influence of external electric fields.