By Dr. John J. Correia and Dr. H. William Detrich, III (Eds.)
Pushed partially by means of the improvement of genomics, proteomics, and bioinformatics as new disciplines, there was a big resurgence of curiosity in actual ways to examine macromolecular constitution and serve as within the context of residing cells. This quantity in equipment in cellphone Biology is dedicated to biophysical strategies in vivo and their purposes to mobile biology. the quantity covers methods-oriented chapters on primary in addition to state of the art innovations in molecular and mobile biophysics. This ebook is directed towards the wide viewers of phone biologists, biophysicists, pharmacologists, and molecular biologists who hire classical and glossy biophysical applied sciences or desire to extend their services to incorporate such ways. it is going to additionally curiosity the biomedical and biotechnology groups for biophysical characterization of drug formulations sooner than FDA approval. * Describes options within the context of vital organic difficulties * Delineates severe steps and power pitfalls for every strategy
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Additional resources for Biophysical Tools for Biologists, Volume Two: In Vivo Techniques
Measuring unfolding of proteins in the presence of denaturant using fluorescence correlation spectroscopy. Biophys. J. 88, 1413–1422. Chen, H. , Butler, J. , Loh, S. , and Webb, W. W. (2007). Dynamics of equilibrium structural fluctuations of apomyoglobin measured by fluorescence correlation spectroscopy. Proc. Natl. Acad. Sci. USA 104, 10459–10464. , Muller, J. , Ruan, Q. , and Gratton, E. (2002). Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. Biophys.
W. F. is supported by NIH Training Grant No. 2-T32-GM007469. We also thank Mark Williams for help with proofreading and editing the text. References Adams, S. , Campbell, R. , Gross, L. , Martin, B. , Walkup, G. , and Tsien, R. Y. (2002). New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: Synthesis and biological applications. J. Am. Chem. Soc. 124, 6063–6076. Alberts, B. (2002). ’’ Garland Science, New York. Aragon, S. , and Pecora, R. (1975). Fluorescence correlation spectroscopy and brownian rotational diVusion.
Chem. Phys. 64, 1791–1803. , Devaux, P. , and Cribier, S. (2000). Translational diVusion of globular proteins in the cytoplasm of cultured muscle cells. Biophys. J. 78, 901–907. Axelrod, D. (2008). Total internal reflection fluorescence microscopy. In ‘‘Biophysical Tools for Biologists, Volume 2: Methods In Vivo’’ (J. J. Correia, and H. W. ), pp. 461–476. Academic Press, San Diego. , Koppel, D. , and Webb, W. W. (1976a). Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.