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And Galill, G (1993) Bacterial dihydrodipicolinate synthase and desensitized aspartate kmase: Two novel selectable markers for plant transformation. Biotechnology 11,7 15-7 18. 59 Goddijn, 0. , Schilperoort, R A, and Hoge, H. C. (1993) A chimaenc tryptophan decarboxylase gene as a novel selectable marker m plant cells Plant Mol Blol. 22,907-9 12 60. , and Caboche, M. (1991) Constitutive nitrate reductase: A dominant conditional marker for plant genetics Plant J 1,267-274. 61. Perera, R J , Linard, C G , and Signer, E R (1993) Cytosine deammase as a negative selective marker for Arabzdopszs Plant Mol.
56-164. , and Wrllmrtzer, L. (1987) Organ specific and dosage dependent expression of a leaf/stem specific gene from potato after tagging into potato and tobacco plants. Nucleic Acids Res. 15,347%3491 30 Deroles, S C and Gardner, R. C. (1988) Expression and inherrtance of kanamycin resistance m a large number of transgemc petunias generated by Agrobacterrum mediated transformanon Plant Mol Blol 11,355364. 3 1. Shnsat, A. , and Croy, R. R. R. (1989) Gene copy number and levels of expression in transgemc plants of a seed specific gene.
7 mL of 15% sodium hypochlortte solution with autoclaved water in a stertle umversal container, add 4 PL Tween 20, shake gently, and use the same day. 3. Media for Growth of Bacteria 1. 8 and volume to 1 L, autoclave. 2. YMA medium for R. loti plates* Add 2 g manmtol and 15 g agar to 1 L of YB medium, autoclave. 3. 0, and volume to 1 L, autoclave. 4. LA medmm for Agrobacterzum and E. colz plates: Add 14 g of agar to 1 L of LB before autoclavmg. Agrobac terium rhizogenes and Higher Plants 53 5. B&D plant growth medium (17): Five millihters of four stock solutions, A, B, C, and D, are mixed with 10 L of water.