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Extra resources for Atomic Force Microscopy: Biomedical Methods and Applications (Methods in Molecular Biology Vol 242)
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No. 430599). The preferred dishes have been pretreated by the manufacturer so as to present a negatively charged and hydrophilic surface and thus ensure better cell adherence. The cells are then washed, as in the previous paragraph, and covered to a depth of 2–3 mm with serumfree media. 3. Fixed Cells The preparation follows a modified procedure of Pietrasanta et al. (6). 1 mM ethylenediamine tetraacetic acid). 01% gluteraldehyde (in S buffer) for 10 min. This is followed by another wash in PBS before being thoroughly rinsed in deionized and distilled water.