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Atomic Force Microscopy: Biomedical Methods and Applications by Davide Ricci, Pier Carlo Braga

By Davide Ricci, Pier Carlo Braga

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Extra resources for Atomic Force Microscopy: Biomedical Methods and Applications (Methods in Molecular Biology Vol 242)

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10. , and Semenza, G. (1996) Nhydroxysuccinimide ester functionalized self-assembled monolayers for covalent immobilization of biomolecules on gold. Biophys. J. 70, 2052–2066. 11. Stoney, G. G. (1909) The tension of metallic films deposited by electrolysis. Proc. R. Soc. London Soc. A 82, 172–175. 12. , et al. (2002) Multiple label-free biodetection and quantitative DNA-binding assays on a nanomechanical cantilever array. Proc. Natl. Acad. Sci. USA 99, 9783–9788. 13. , et al. (2003) A label-free protein assay based on a nanomechanical cantilever array.

Datar, R. , Hansen, K. , Cote, R. , and Majumdar, A. (2001) Bioassay of prostate-specific antigen (PSA) using microcantilevers. Nat. Biotech. 19, 856–860. 17. , O’Connor, G. , et al. (2001) Characterisation of an antibody coated microcantilever as a potential immuno-based biosensor. Biosens. Bioelectron. 17, 201–207. 18. , Meyer, E and Christoph Gerber (2002) Nanomechanics from atomic resolution to molecular recognition based on atomic force microscopy technology. Nanotechnology 13, R29–R36. 50 Hegner and Arntz Analysis of Human Fibroblasts by AFM 53 5 Analysis of Human Fibroblasts by Atomic Force Microscopy Gillian R.

No. 430599). The preferred dishes have been pretreated by the manufacturer so as to present a negatively charged and hydrophilic surface and thus ensure better cell adherence. The cells are then washed, as in the previous paragraph, and covered to a depth of 2–3 mm with serumfree media. 3. Fixed Cells The preparation follows a modified procedure of Pietrasanta et al. (6). 1 mM ethylenediamine tetraacetic acid). 01% gluteraldehyde (in S buffer) for 10 min. This is followed by another wash in PBS before being thoroughly rinsed in deionized and distilled water.

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