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Advances in Genetics, Vol. 23 by E.W. Caspari, John G. Scandalios (Eds.)

By E.W. Caspari, John G. Scandalios (Eds.)

This quantity in a chain on genetics, emphasizes the variety of genetic experiences. Articles hide the filamentous fungus neurospora, biogenesis of yeast ribosomes, evolutionary genetics of fish, drosophila transposable components and the dropophila gene zeste.

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Pornbe 972 h - as a function of UV fluence (A) or 6OCo y-ray exposure (B). The specific survival response toward the two DNA-damaging agents is thought to reflect the repair capabilities in relation to the presence of one or two copies of the genome at the time of the damage. From Gentner (1981). bimodal shape of the inactivation curves of asynchronous cultures (Fabre 1970; Gentner and Werner, 1975). From these biphasic curves, which describe the heterogeneity of the population, a resistant part emerges representing the relative amount of G, cells in the analyzed culture (Fig.

1977, 1983; Loprieno, 1981; Loprieno and Abbondandolo, 1980). AND MUTATIONAL SYSTEMS A. MUTANTENRICHMENT Several techniques have been developed for mutant enrichment. Nutrient limitation has been used to enhance mutation frequencies (McAthey and Kilbey, 1976, 1978). Inositol starvation inducing inositol-less death of the nonmutated cells upon transfer of the population onto complete media has been developed by Megnet (1964) and described in Gutz et al. (1974). Similarly, a 2-deoxyglucose procedure of enrichment has been developed (Megnet, 1965).

RADIOSENSITIVE MUTANTS The identification of two major pathways for repair in S. pombe has been established by the isolation of radiosensitive mutants and by the observation that caffeine is a potent inhibitor of dark repair in the fission yeast (Fig. 2). The need to search for new radiosensitive strains has already been stressed by Nasim and Auerbach (1967). The authors used such strains in an attempt to test the repair hypothesis supported by their data on mosaicism (Nasim and Auerbach, 1967).

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