By Richard B. Thompson (auth.), Chris D. Geddes, Joseph R. Lakowicz (eds.)
Topics in Fluorescence Spectroscopy, complicated thoughts in Fluorescence Sensing half B: Macromolecular Sensing, is the 10th quantity within the well known sequence Topics in Fluorescence Spectroscopy, edited through Drs. Chris D. Geddes and Joseph R. Lakowicz.
This quantity contains authoritative macromolecular analytical fluorescence sensing stories really good adequate to be beautiful to specialist researchers, but additionally attractive to the broader viewers of scientists in similar disciplines of fluorescence. This quantity at once compliments quantity nine within the sequence, Part A: Small Molecule Sensing.
Advanced techniques in Fluorescence Sensing half B: Macromolecular Sensing is an important reference for any lab operating within the analytical fluorescence sensing box. All lecturers, bench scientists, and execs wishing to use the most recent and maximum within the regularly rising box of macromolecular fluorescence sensing will locate it a useful resource.
Topics in Fluorescence Spectroscopy, complicated recommendations in Fluorescence Sensing half B: Macromolecular Sensing subject matters include:
Protein-based Biosensors with Polarization established Transduction
GFP Sensors
Fluorescent Saccharide Sensors
Fluorescent Pebble Nano-Sensors
Aptamers for Macromolecular Sensing
Molecular Imprinting
Excimer Sensing
About the Editors:
Chris D. Geddes is an affiliate Professor of Fluorescence Spectroscopy, Director of the Institute of Fluorescence, and affiliate Director of the heart for Fluorescence Spectroscopy on the college of Maryland Biotechnology Institute, scientific Biotechnology middle, Baltimore. he's the Editor-in-Chief of the Journal of Fluorescence and either the Editor-in-Chief and Founding Editor of the Who's Who in Fluorescence and Reviews in Fluorescence annual volumes, in addition to government Director of the Society of Fluorescence. Dr Geddes has released over a hundred medical articles, papers, evaluation articles, books and booklet chapters at the ideas and functions of fluorescence.
Joseph R. Lakowicz is Professor of Biochemistry on the college of Maryland tuition of drugs, Baltimore, and Director of the guts for Fluorescence Spectroscopy. Dr. Lakowicz has released over four hundred medical articles, has edited various books, holds sixteen issued patents, and is the writer of the commonly used textual content, ideas of Fluorescence Spectroscopy.
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Additional info for Advanced Concepts in Fluorescence Sensing: Part B: Macromolecular Sensing
Sample text
Upon phosphorylation of the kinase consensus sequence, the phophoamino-binding domain induced conformational changes in the linker domain. For example, a PKA sensor was engineered using CFP and citrine joined by the 14-3-31 phosphoserine/threonine binding domain and a 21 amino acid kinase substrate domain (22). The maximum change in emission ratio for this probe was ",-,30 % for physiological stimuli in vivo. Sensors for the specific tyrosine kinase activities of Src, Abl, epidermal growth factor (EGF) receptor, and insulin signaling have been generated using linkers composed of Src homology (SH) 2 domains (to bind phosphotyrosine) and specific kinase consensus substrates (93, 94).
White, C. , & Argauer, R. J. (1970). Fluorescence Analysis: A Practical Approach. New York: Marcel Dekker, Inc. , Reed, J. , FeIgner, P. , & Corbeil, J. (2001). Intracellular delivery ofproteins with a new lipid-tnediated delivery system. Journal ofBiological Chemistry, 276(37),35103 - 35110. GFPSENSORS Peter M. S. 1. INTRODUCTION The green fluorescent protein (GFP) and related genetically-encoded fluorescent proteins have had a major impact in cell biology. GFP has diverse applications in studies of protein localization, dynamics, interactions and regulation.
295 - 334). New York: Plenum. , & Lakowicz, J. R. (1995). Fluorescence polarization imtnunoassay of a high molecular weight antigen based on a long lifetime Ru-ligand complex. Analytical Biochemistry, 227, 140147. Thompson, R. , Patchan, M. , & Fierke, C. A. (1996). Performance enhancetnent of fluorescence energy transfer-based biosensors by site-directed mutagenesis of the transducer. Journal of Biomedical Optics, 1(1),131-137. Thompson, R. , & Jones, E. R. (1993). Enzytne-based fiber optic zinc biosensor.